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Faculty

Lisa Lapidus, Assistant Professor

Lisa LapidusE-mail:
Website: www.pa.msu.edu/people/lapidus/
Research Description PDF

Education

B.S. 1991 University of Michigan
A.M. 1993 Harvard University
Ph.D. 1998 Harvard University

 

 

Research Focus

Biological Physics, Protein Folding

The Lapidus lab studies protein folding using optical spectroscopy and microfluidics. We currently have the fastest continuous flow mixer in the world which can mix two solutions and prompt protein folding in ~4 microseconds. We are currently developing new mixers to improve mixing time and apply microfluidics to new spectroscopic methods.

The Lapidus lab also studies the early stages of folding and the dynamics of unfolded proteins. We recently showed that intramolecular diffusion of unfolded protein L is 1000 times slower than previously estimated. This finding may profoundly change the way protein folding theories are constructed because the search for native structure may be limited by this slow reconfiguration. We are also looking at how the rates of intramolecular diffusion depends on sequence and have found that this rate is related to the propensity for a protein to aggregate.

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Selected Publications

Y. Chen, W.J. Wedemeyer, L.J. Lapidus, A General Model of Unfolded Proteins under Folding Conditions. J. Phys. Chem. B. (submitted).

S. A. Waladauer, O. Bakajin, L.J. Lapidus, Extremely Slow Intramolecular Diffusion in Unfolded Protein L, Proceedings of the National Academy of Sciences (in press).

V.A. Voelz, V.R. Singh, W.J. Wedemeyer, L.J. Lapidus, V.S. Pande, Unfolded state dynamics and structure of protein L characterized by simulation and experiment. Journal of the American Chemical Society, 132, 4702-4709 (2010).

Y. Chen, C. Parrini, N. Taddei, L.J. Lapidus, Conformational Properties of Unfolded HypF-N, J. Phys. Chem. B 113, 16209-16213 (2009).

S. DeCamp, S.A. Waldauer, A. Naganathan, O. Bakajin, L.J. Lapidus, Direct Observation of Downhill folding of l-repressor in a Microfluidic Mixer, Biophys. J. 97, 1772-1777 (2009).